Austria – Txiv hmab txiv ntoo thiab Grapevines – tus kab mob no kuj-BOKU Austria

Tom qab cov ntaub ntawv thiab cov ntsiab

Cov mov av nepovirus Grapevine Fanleaf kab (GFLV) ua rau tus kab mob fanleaf grapevine thiab txiv moj mab qoob Potyvirus (PPV) kis zeb txiv ntoo ua tus kab mob Sharka. Tiv thaiv ntawm tus kab mob vectors nyob hauv nroj tsuag ntxhov xws li tshua inefficient los yog raug txwv vim cov dejnum los ntawm cov tshuaj tua kab nyob rau ntawm tej. Qhia rau tus economic tseem ceeb ntawm cov kab mob no, ntau tau npaj siab hu mus after kuj los ntawm kev taw qhia txog noob CP.

Thiaj li tsim resistant apricots, txiv ntoo qab zib, txiv moj mab thiab grapevines tus tiv thaiv npaum nkaus xwb tsis, tiam sis kuj sib nrauj tej kev nyab xeeb tau xam. Kev txhawj xeeb tau tau tsa hais txog tej Conference noob transgenic hlav. Txawm tias tej kev txhawj xeeb tsim nyog soj attentive, xwb seb puas yog cov ntaub ntawv nyob hauv lub step-by-step kev yuav pub judging cov nqi ntawm cov qoob loo.

Lawm seb puas raug tshav nyob hauv Austria cov ntaub ntawv ntawm ib leej molecular characterization lub nyoog sib txawv yog ib prerequisite, qhov no yog tiav blot yav qab teb thiab lam analyses, neb ob leeg T-DNA thiab caj qaum vector sequences.

Transgene instability muaj lawm pheej nov lawd ua phiv nyhuv ntev. Nyob rau hauv peb cov kev tshawb fawb qhia lub hauv nroj tsuag ntxhov, xws li txiv hmab txiv ntoo pob zeb, zoo li yuav ruaj khov hauv vitro nyob rau hauv thiab nyob rau hauv vivo, ob leeg nyob rau hauv lub tsev cog khoom thiab lub sij hawm ntev raws li tej yam kev mob screenhouse.

Kev txiav txog tus nqi rau kab transgenic yuav muab tau tsuas yog tus kab mob no raug saib, tus tiv thaiv phenotype exhibits uas cov kab, Thaum tuav nthuav lub sijhawm agronomic.

Ntawm txoj kev loj hlob

Hauv vitro assays teg (lab), nyob rau hauv planta assays lub tsev cog khoom thiab cov screenhouse muaj ntse muaj kev soj ntsuam.

Vim li cas delaying, diverting los siv ceev xwmphem txoj kev soj ntsuam

Teb thwmsim nrog kab noob hnov txog cov txiv hmab txiv ntoo thiab resistant tiv thaiv hom kab mob grapevines (PPV thiab GFLV) yuav tsis tau ua, vim yog lub hurdles ntawm kev txhaum, e.g. daim ntawv kho mob yuav tsum.

Cov kev pab cuam foregone

Tus sau qoob txhob kaw engineered txiv hmab txiv ntoo thiab grapevines nrog rau cov kab mob ua hauj yuav tshuam kom siv cov tshuaj tua kab tsuag hauv tej qoob loo, saib dua kom muaj nuj nqis thiab zoo yields, txawm nyob hauv siab siab yav. Qhov no yuav muaj lig ib feem tso nyiaj ncaj qha rau ntawm qhov chaw, neeg kho mob, cov nqi ntau lawm thiab profitability ntawm cov qoob loo. Organic tswv teb yuav tshwj xeeb tshaj yog pab los ntawm cov nroj tsuag, vim lawv yuav tswj kev siv tau thiab tsim nyog sim kev tiv thaiv thiab txo qhov uas xav tau cov tshuaj tua kab hluavtaws.

Ntxiv rau hauv general, kev siv ntawm cov nroj tsuag yuav ua kuas zus ntawm cov txiv hmab txiv ntoo thiab grapevines nyob teb chaws Europe tau yav tom ntej, tswj cov nyhuv lawm (positive) ntawm kev txuag toj roob hauv pes thiab txo qhov uas cov dependence rau cov zaub mov imports.

Nqi ntawm cov kev tshawb fawb

Cov kev soj ntsuam kev tab tom nyob rau lub caij 1980ies yeej txaus siab los ntawm Austrian Ministries BMWF thiab BMLFUW, tus EU thiab lub University of Natural Resources thiab Applied Life Sciences (BOKU) muaj ib tug nqi ~1.587.000 €.

Duab

Chiv keeb

Maghuly F., Khan M.A., Borroto Fernandez E., Druart P., Bernard Watillon B., Laimer M. 2008. Kev nyuab siab uas qhia txog cov GUS-marker noob (uidA) nyob qab tswjhwm ntawm tsob nroj calmodulin thiab 35S tus promoters hauv ib hom txiv hmab txiv ntoo rootstock: Prunus incisa x serrula. J. Biotechn. 135: 105-116

Prins M., Laimer M., E. noris, J. Schultz, Wassenegger M., Tepfer M. 2008. Tswv yim rau cov tshuaj siv los kho kuj nyob transgenic nroj tsuag. Mol. Cog Pathol. 9: 73-83.

Laimer M. 2007. Transgenic grapevines. Transgenic cog Journal 1 (1): 219-227.

Maghuly F., da Câmara Machado A., Leopold S., Khan M.A., Katinger H., Laimer M. 2007. Ntev stability ntawm marker noob qhia rau Prunus subhirtella: Ib hom txiv hmab txiv ntoo hom. J. ntawm Biotechn. 127: 310-321.

Laimer M. 2006. Tus kab mob no kuj yuav nteg qe rau cov txiv hmab txiv ntoo. Nyob rau hauv: Ntoo transgenic. Fladung M. thiab Ewald D. eds. Springer. 181-199.

Maghuly F., Leopold St., da Câmara Machado A., Borroto Fernandez E., Khan M.A., Gambino G., Gribaudo kuv., Schartl A., Laimer M. 2006. Molecular characterization grapevine hlav muaj cov noob ua hauj GFLV: II. Tsev kawmntawv Cell tsob nroj. 25: 546-553.

Laimer M., Mendonça D., Myrta A., Boscia D., Katinger H. 2005. Isolates molecular characterization of Austrian PPV. Phytopath. Polonica 36: 25 – 32.

Laimer M., Mendonça D., da Câmara Machado A., Maghuly F., Khan M., Katinger H. 2005. Kuj yuav nteg qe rau PPV hauv Austria: Lub xeev ntawm cov kos duab. Phytopath. Polonica 36: 97 – 105.

Gambino G., Gribaudo kuv., Leopold St., A Schartl. thiab Laimer M. 2005. Molecular characterization grapevine hlav muaj cov noob ua hauj GFLV: Kuv. Ntaub ntawv ntawm tsob nroj 24: 655 -662.

Laimer M. 2005. Lub biotechnology tshab mus ntsib nws hnub no txoj kev sib tw. Fifth Vienna Globalization Symposium. 13. – 14. 5. 2004. 227 – 232.

Laimer M., Mendonça D., Maghuly F., Marzban G., Leopold S., Khan M., Kirilla Z., Balla kuv. thiab Katinger H. 2005. Biotechnology ntawm cov ntoo txi txiv tsis kub tsis txias. Acta Biochim. Polon. 52 (3): 673-678.

Laimer M. 2004. Kev sis caav GMO: Cov lus teb nyob sab Europe. Ntaub ntawv uas qhia txog qhov 4. Sib tham nrog rau American-lwm tebchaws koom tes rau hauv khoom noj khoom haus thiab Agricultural kev kawm thiab kev tshawb kawm transatlantic. C. Karssen (Ed.) Beauvais, 2. – 3. Tim 2003. 80-89.

Laimer M. 2003. Characterisation transgenic cov txiv hmab txiv ntoo thiab analyses rawg lom kev. Nyob rau hauv: Conference feem ntawm GMO nthuav rau Agro-Ecosystems. (eds. Lelley T., Balázs E. thiab Tepfer M.) Facultas, Vienna: 101-113.

Laimer M., Mendonça D., Arthofer W., Hanzer V., Myrta A., Boscia D. 2003. Tshwm sim rau lub txawv saum qoob hlwv dej no hom rau ntau hom txiv hmab txiv ntoo pob zeb nyob hauv Austria. Opt. Médit. Sér. B 45: 79-83.

Laimer M. 2003. Ej transformation ntawm cov qoob loo tsis kub tsis txias ntxhov txiv ntoo. Nyob rau hauv: Laimer M. thiab Rücker W. 2003. Cog nqaij Culture: 100 xyoos txij Gottlieb Haberlandt. Springer Verlag, Wien: 217-242.

Minafra A., Goelles R., da Câmara Machado A., Saldarelli P., Buzkan N., Savino V., Martelli G.P., Katinger H., Machado Laimer da Câmara M. 1998. Qhia txog lub tsho tiv no protein ntau noob kab mob grapevine A thiab B nyob hauv Nicotiana, thiab qhov kev luj xyuas ntawm tus hauj conferred los transgenic nroj tsuag. J. kev cog Pathol. 80:197-202.

Gölles R., da Câmara Machado A., Tsolova V., A. pob paj, Moser R., Katinger H., Machado Laimer da Câmara M. 1997. Transformation rau somatic embryos ntawm Vitis sp. nrog lwm constructs uas muaj nucleotide sequences los nepovirus lo protein ntau noob. Acta Hort. 447: 265-272.

da Câmara Machado A., Puschmann M., Pühringer H., Kremen R., Katinger H., Machado Laimer da Câmara M. 1995. Somatic embryogenesis Prunus subhirtella autumno-rosa thiab lwm cov zaub transgenic tom qab transformation Agrobacterium-mediated. Tsev kawmntawv Cell tsob nroj. 14: 335-340.

Laimer da Câmara Machado M., da Câmara Machado A., Hanzer V., Weiß H., Regner F., Steinkellner H., Mattanovich D., Plail R., Knapp E., Kalthoff B., Katinger H. 1992. Regnereration transgenic hlav ntawm Prunus armeniaca uas muaj lub tsho tiv no protein ntau noob uas tus kab mob qoob hlwv dej txiv moj mab. Tsev kawmntawv Cell tsob nroj. 11: 25-29

Saib tus neeg taug lw

Margit Laimer, Pflanzenbiotechnologie Gruppe, IAM, Koom Biotechnologie, Universität für Bodenkultur (BOKU), Muthgasse 18, A-1190 Vienna, Austria

Kev sib cuag

m.laimer@iam.boku.ac.at

Ntxiv neeg ua tim khawv

http://www.biotec.boku.ac.at/pbu.html

http://www.boku.ac.at/sicherheitsforschung/Open-e.htm