배경 정보 및 관련 결과
The aim of the present study was to assess possible adverse effects of transgene expression in leaves of field-grown barley relative to i) the influence of genetic background and ii) effect of plant interaction with arbuscular mycorrhizal fungi. We conducted parallel transcript profiling, metabolome profiling and metabolic fingerprinting of wild-type accessions and barley (Hordeum vulgare L.) transgenics with i) seed-specific expression of (1,3-1,4)-ß-glucanase (GluB) being introgressed from the cultivar Golden Promise (GP) into the cultivar Baronesse (B) as well as of transgenics in the GP background with ii) ubiquitous expression of codon-optimized Trichoderma harzianum endochitinase (ChGP) that we generated and characterized in the course of the study.
We found more than 1600 differential transcripts between the varieties GP and B, with defense genes being strongly overrepresented in B, indicating a divergent response to subclinical pathogen challenge in the field. In contrast, no statistically significant differences between ChGP and GP could be detected based on transcriptome or metabolome analysis.
개발의 단계
온실 및 실험실 분석. 필드 시험 단계.
블록 / 지연 이유
지난 2 년 동안, the incidence of scientific field trials being vandalised and destroyed by radical anti-biotechnology activists has drastically increased. 이 글은 기록을 명중 2009, 와 42% 필드 사이트에서 고가의 보안 및 감시 조치에도 불구하고 - 독일 현장 시험의 파괴, 일반 대중에게 알리는 과학자 광범위한 커뮤니케이션 노력, 이전과 방출 실험을하는 동안 모두.
Our field trials were the target of activist groups in 2006, 2007, 2008, 과 2009. The destruction was always up to 30% of plants. 에 2009, after the complete destruction, we could save the experiment only upon complete resowing.
Futhermore, due to political unclearities it was always difficult to get the final approval for the sowing, although the general approval for the biosafety project and field release had been granted. Especially since 2008 it was very difficult to get the final go for the release. For instance in 2009, we experienced a dramatic delay resulting in a late sowing of barley on May 12 (optimal barley sowing is at the end of March until early April).
필연적 인 혜택
The cost for the experiments increased dramatically due to the fact that we needed to protect field sites day and night during the whole growing period. Due to the destructions the project was prolonged for 2 년. Important scientific goals of the project could not be reached, including some epidemiologic studies related to the resistance status of genetically modified barley plants.
The barley is resistant to devastating Rhizoctoni solani root rot. Using this barley could reduce pesticides and by the same time support ecologically desirable reduced-tillage strategies that save soil structure and microbial communities.
사진
Genetically modified barley plants on the field site
연구의 비용
Ca. 500.000 €
출판에 대한 참조
책임 연구원
Karl-Heinz Kogel, Institut für Phytopathologie und Angewandte Zoologie, Justus-Liebig-Universität Gießen, Heinrich-Buff-Ring 26, D-35392 Gießen, 독일
연락처 정보
Karl.-Heinz.Kogel@agrar.uni-giessen.de