Zenk Nucleases Dwèt (ZFN)

koreksyon genomic se egzak modifikasyon an vize nan sekans lan nukleotid nan genomic a.

Pandan dènye dekad ADN nan pwogramasyon obligatwa pwoteyin te devlope ki ka gide yon nukleaz nan koupe nan nenpòt ki pwen vize nan genomic a.

The Zinc Finger Nuclease (ZFN) technique uses natural proteins that bind to DNA in a sequence-specific way, sa ki pèmèt nukleaz nan kole nan koupe kòm yon “sizo ADN’ nan ki kote espesifik.

Sistèm sa a konsiste de:

• a “Zinc Finger” domain (recognising a sequence of specific base pairs triplets in the DNA sequence)
• yon nukleaz ki koupe doub-bloke ADN

Lyen

• EFSA Scientific opinion addressing the safety assessment of plants developed using Zinc Finger Nuclease 3 and other Site-Directed Nucleases with similar function (2012)

Method of the Year 2011: Gene-editing nucleases – by Nature Video