editjar Ġenoma huwa l-modifikazzjoni preċiża immirat tas-sekwenza tan-nukleotidi tal-ġenoma.
Fil-każ ta 'CRISPR / Cas9, gwida RNA jieħu l-post ta 'proteini tad-DNA, therefore simplifying the process. CRISPR stands for “Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)”.
The CRISPR/Cas9 system is based on a bacterial defense system against foreign DNA (e.g. viruses), whereby an RNA guided nuclease makes highly targeted cuts in the genome.
The CRISPR-Cas9 complex consists of (ara l-istampa hawn taħt)
- a Cas9 protein (Cas9 stands għall “CRISPR assoċjat)
- a RNA gwida waħda (sgRNA)
Links:
- https://geneticliteracyproject.org/category/gene-editing/?mc_cid=2335049bf7&mc_eid=0d9495ef74
- Real-ispazju u fil-ħin reali dinamika tal CRISPR-Cas9 viżwalizzati bl b'veloċità għolja mikroskopija seħħ atomika (2017-11)
- L Video Zaskakuje Shows CRISPR Editjar DNA fi Żmien Reali (2017-11)
- Użu ta 'sistemi CRISPR fl-editjar ġenoma tal-pjanti, Ricroch et al 2017
- mutanti Menu
- http://www.arte.tv/guide/en/063945-025-A/gene-editing-in-plants
- valutazzjoni allerġeniċità ta 'proteina Cas9 ssib mhuwiex probabbli li ma għandu jkun allerġenu ikel (2017)
- Vox: Gwida sempliċi biex CRISPR, waħda mill-istejjer xjenza akbar ta ' 2016
- DNA editjar ġenoma ħieles tal-qamħ – 2016 – 12
- Qamħ CRISPR DNA temporanji jew espressjoni RNA – 2016 – 04
- Ġenoma Editjar ma CRISPR-Cas9
- Jennifer Doudna (UC Berkeley / HHMI): Inġinerija ġenoma ma CRISPR-Cas9
- http://www.nature.com/news/crispr-gene-editing-is-just-the-beginning-1.19510
- http://genomebiology.biomedcentral.com/articles/10.1186/s13059-015-0826-7
- https://www.youtube.com/watch?v = MnYppmstxIs&app = desktop
- Titanic kunflitt fuq il-patenti CRISPR dawriet ikrah, http://www.nature.com/news/titanic-clash-over-crispr-patents-turns-ugly-1.20631
- agrikola R&D huwa fuq il-moviment, http://www.nature.com/news/agricultural-rd-is-on-the-move-1.20571
