Kawm ntawv kuas Nuclease (SDN) genome editing yuav siv ntau DNA-tej enzymes (nucleases) Uas yog qhia txiav cov DNA ntawm ib qhov chaw predetermined los ntawm ntau hom DNA binding lub nruab. Tom qab txiav yog txiav, Ntawm tes tus kheej DNA kho mechanism paub txog cov so thiab kho puas tsuaj, Siv ib ob pathways uas yog lawm tam sim no nyob rau hauv hlwb:
- Tsis-homologous kawg-pub ntxiv tshaj (NHEJ): Qhov ntuag DNA yog rejoined, Tab sis thaum ua qhov no ob peb basepairs tej zaum yuav noj tam sim ntawd los yog ntxiv uas ua rau random me deletions (nce 20) ntxiv (ob peb basepairs) Ntawm nucleotides ntawm qhov ntuag qhov chaw.
- homology-qhia kho (HDR): Ib donor DNA uas yog muaj siab hloov thiab muaj homology nrog lub hom phaj yog siv los ua kom paub qhov kev hloov no hloov ntawm qhov chaw txiav. Ua li no koj yuav ua kom paub meej tias intentional insertions, kev hloov los yog deletions.
Genome editing tso cai rau cov intentional thiab precise knock tawm hauv ib undesirable trait los yog cov (rov qab ua dua)Kev taw qhia txog ib tug muaj siab trait.
Nuclease mediated genome editing txheej txheem muaj xws li: