Genome editing yog tus leej ntxiv yog hloov tus lawv liag nucleotide lub genome.
Tus CRISPR/Cas9, ib daim ntawv qhia RNA yuav siv qhov chaw uas muaj protein ntau DNA ruaj, yog li ntawd simplifying txoj kev. CRISPR stands “Clustered tsis tu ncua Interspaced Palindromic Repeats luv luv (CRISPR)”.
CRISPR/Cas9 uas yog los ntawm ib tug kws muaj txuj ci rau txawv teb chaws DNA (e.g. kab mob), whereby ua ib RNA nuclease ua ntxiv mas to thiab nyob hauv lub genome.
Lub CRISPR-Cas9 complex muaj (saib duab hauv qab no)
- muaj protein ntau Cas9 (Cas9 es mus “Mob CRISPR)
- ib qho kev qhia RNA (sgRNA)
Kev mus:
- https://geneticliteracyproject.org/category/gene-editing/?mc_cid=2335049bf7&mc_eid=0d9495ef74
- Puab ntawm chaw seem thiab real-time kev CRISPR-Cas9 visualized ntawm cov hwjchim high-speed atomic microscopy (2017-11)
- Kev yees duab Astonishing qhia CRISPR Editing DNA ntawm sij hawm (2017-11)
- Siv lub nruab CRISPR rau tsob nroj genome editing, Ricroch li al 2017
- Mutant zaub mov
- http://www.arte.tv/guide/en/063945-025-A/gene-editing-in-plants
- Kev txheeb xyuas allergenicity Cas9 protein pom nws tsi muaj tej zaum yuav muaj zaub mov allergen (2017)
- Vox: Ib daim qhia yoog mus CRISPR, ib tug biggest zajlus kev kawm txog 2016
- DNA dawb genome editing hnav – 2016 – 12
- Nplej CRISPR nraum DNA lossis RNA qhia – 2016 – 04
- Genome Editing nrog CRISPR-Cas9
- Jennifer Doudna (UC Berkeley / HHMI): Genome Engineering nrog CRISPR-Cas9
- http://www.nature.com/news/crispr-gene-editing-is-just-the-beginning-1.19510
- http://genomebiology.biomedcentral.com/articles/10.1186/s13059-015-0826-7
- https://www.youtube.com/watch?v = MnYppmstxIs&APP = desktop
- Titanic clash nyob CRISPR patents puv dab tuag, http://www.nature.com/news/titanic-clash-over-crispr-patents-turns-ugly-1.20631
- Agricultural R&D yog nyob qhov, http://www.nature.com/news/agricultural-rd-is-on-the-move-1.20571
