Genome editing is the precise targeted modification of the nucleotide sequence of the genome.
In the case of CRISPR/Cas9, a guide RNA takes the place of a DNA binding protein, therefore simplifying the process. CRISPR stands for “Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)”.
The CRISPR/Cas9 system is based on a bacterial defense system against foreign DNA (e.g. viruses), whereby an RNA guided nuclease makes highly targeted cuts in the genome.
The CRISPR-Cas9 complex consists of (see picture below)
- a Cas9 protein (Cas9 stands for “CRISPR associated)
- a single guide RNA (sgRNA)
Links:
- https://geneticliteracyproject.org/category/gene-editing/?mc_cid=2335049bf7&mc_eid=0d9495ef74
- Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy (2017-11)
- An Astonishing Video Shows CRISPR Editing DNA in Real Time (2017-11)
- Use of CRISPR systems in plant genome editing, Ricroch et al 2017
- Mutant Menu
- http://www.arte.tv/guide/en/063945-025-A/gene-editing-in-plants
- Allergenicity assessment of Cas9 protein finds it is not likely to be a food allergen (2017)
- Vox: A simple guide to CRISPR, one of the biggest science stories of 2016
- DNA free genome editing of wheat – 2016 – 12
- Wheat CRISPR transient DNA or RNA expression – 2016 – 04
- Genome Editing with CRISPR-Cas9
- Jennifer Doudna (UC Berkeley / HHMI): Genome Engineering with CRISPR-Cas9
- http://www.nature.com/news/crispr-gene-editing-is-just-the-beginning-1.19510
- http://genomebiology.biomedcentral.com/articles/10.1186/s13059-015-0826-7
- https://www.youtube.com/watch?v=MnYppmstxIs&app=desktop
- Titanic clash over CRISPR patents turns ugly, http://www.nature.com/news/titanic-clash-over-crispr-patents-turns-ugly-1.20631
- Agricultural R&D is on the move, http://www.nature.com/news/agricultural-rd-is-on-the-move-1.20571
